Rat brain-derived neurotrophic factor (rT BDNF) ELISA quantifies rT BDNF in rat serum, plasma, or cell culture medium. The assay will specifically recognize both natural and recombinant RT BDNF.
BDNF Rat Sandwich Solid-Phase ELISA (Enzyme-Linked Immunosorbent Assay) was developed to measure the number of targets bound between a matching antibody pair. The specific target antibody is pre-coated into the provided microtiter plate hole. Several companies also make the Human MAG ELISA Kit available online.
The sample, standard, or control is then added to these wells and bound to the immobilized antibody. The sandwich is made by adding a second antibody (detector), adding a substrate solution that reacts with the enzyme-antibody target complex to produce a measurable signal.
Each batch produced from this ELISA kit is quality checked based on criteria such as sensitivity, specificity, accuracy, and consistency between batches. More verification information can be found in the manual.
BDNF (a neurotrophic factor that comes from the brain) belongs to the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage of proBDNF. Although further processing yields a mature 14 kDa protein, pro-BDNF is biologically active and is secreted into synaptic vesicles along with the mature form.
BDNF is widespread in the central nervous system and exerts autocrine and paracrine effects on several classes of neurons. Signal transmission is primarily mediated by the tyrosine kinase receptor TrkB, although binding to the low-affinity receptor, p75-NTR, has also been demonstrated.
BDNF promotes neuronal survival and differentiation and has been shown to play an important role in memory formation and synaptic regulation. BDNF is induced by cortical neurons and is required for the survival of striatal neurons in the brain.